LB-Broth Miller Size:6 x 1kg Tryptone and Yeast are present

Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen

20 X sterile RNAse free stock solution

minus Cysteine

3 gram powdered medium in 1 litre distilled water

during fermentation

LB-Broth Miller Size:6 x 1kg Tryptone and Yeast are presentLB Broth High Salt, Miller's LB Broth, Luria Broth LB Broth Miller is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient