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Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base
The extract is an excellent nitrogen source for bacteria
M9 Minimal Salts Base
A Bromcresol purple solution (0
Molecular cloning: a laboratory manual
Nutrient Broth Size:500g Methionine and Tryptophan concentrations hasFormula g l Peptone 5 Beef Extract 1 Yeast Extract 2 NaCl 5 Final pH 7. 0 0. 2 at 25C Suspend 13 gram powdered medium in 1 litre distilled water Store dry at room temperature
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